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mtt-based colorimetric assay  (Millipore)


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    Millipore mtt-based colorimetric assay
    Mtt Based Colorimetric Assay, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mtt-based+colorimetric+assay/3++4+5+dimethylthiazol+2+yl++2+5+diphenyltetrazolium+bromide++mtt/pm39539274-268-13-21
    Average 90 stars, based on 1 article reviews
    mtt-based colorimetric assay - by Bioz Stars, 2026-09
    90/100 stars

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    MTT Assay:

    Article Title: Pentacyclic triterpenes with nitric oxide inhibitory activity from Potentilla chinensis.
    Article Snippet: Three new ursane-type triterpenes (1–3) and twenty-one known triterpenoids (4–24) were isolated from the methanolic extract of the whole plants of Potentilla chinensis.. Their structures were elucidated by extensive spectroscopic analysis of 1D and 2D NMR (HSQC, HMBC, COSY and ROESY) and HRESIMS data.. The bioassay screening revealed the inhibitory effects on nitric oxide production of compounds 2, 5, 7, 9, 10, and 13–24 in LPS-induced RAW264.7 macrophages.

    Article Title: Jatrophane and ingenane-type diterpenoids from Euphorbia kansui inhibit the LPS-induced NO production in RAW 264.7 cells
    Article Snippet: Accepted Manuscript Jatrophane and ingenane-type diterpenoids from Euphorbia kansui inhibit the LPS-induced NO production in RAW 264.7 cells Jin Woo Lee, Qinghao Jin, Hari Jang, Dongho Lee, Sang Bae Han, Youngsoo Kim, Jin Tae Hong, Mi Kyeong Lee, Bang Yeon Hwang PII: S0960-894X(16)30520-0 DOI: http://dx.doi.org/10.1016/j.bmcl.2016.05.035 Reference: BMCL 23894 To appear in: Bioorganic & Medicinal Chemistry Letters Received Date: 4 January 2016 Revised Date: 29 April 2016 Accepted Date: 12 May 2016 Please cite this article as: Lee, J.W., Jin, Q., Jang, H., Lee, D., Han, S.B., Kim, Y., Hong, J.T., Lee, M.K., Hwang, B.Y., Jatrophane and ingenane-type diterpenoids from Euphorbia kansui inhibit the LPS-induced NO production in RAW 264.7 cells, Bioorganic & Medicinal Chemistry Letters (2016), doi: http://dx.doi.org/10.1016/j.bmcl.. 2016.05.035 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

    Article Title: Chemical constituents from Belamcanda chinensis and their inhibitory effects on nitric oxide production in RAW 264.7 macrophage cells.
    Article Snippet: Belamcanda chinensis (L.) DC., which belongs to the family of Iridaceae, has been used as a folk medicine for the treatment of inflammation, asthma, tonsillitis, and many other throat disorders.. Bioactivity-guided purification of the methylene chloride-soluble fraction of the rhizomes of B. chinensis based on the inhibition of nitric oxide production led to the identification of seventeen known compounds.. Their structures were elucidated on the basis of extensive spectroscopic measurement such as NMR and ESI–MS.

    Article Title: Amaryllidaceae Alkaloids Screen Unveils Potent Anticoronaviral Compounds and Associated Structural Determinants.
    Article Snippet: Cell viability was monitored with the Cell-Titer GLO assay kit (Promega) or the MTT-based colorimetric assay (Cell proliferation Kit I, Roche, Millipore Sigma), where indicated.

    Article Title: Inhibitory constituents of Sophora tonkinensis on nitric oxide production in RAW 264.7 macrophages
    Article Snippet: Bioactivity-guided fractionation of the MeOH extract from the roots of Sophora tonkinensis resulted in the isolation of a new pterocarpan glycoside (1) together with nine known compounds, maackiain (2), sophoranone (3), sophoranochromene (4), pinoresinol (5), syringaresinol (6), medioresinol (7), 40 ,7-dihydroxyflavone (8), calycosin (9), and genistein (10).. The structure of the new compound was determined on the basis of spectroscopic analysis including NMR and CD data in combination with acid hydrolysis.. Compounds 1–4 exhibited the inhibitory effects on LPS-induced nitric oxide production with IC50 values ranging from 13.6 to 33.0 lM in RAW 264.7 macrophages.

    Article Title: Nitric oxide inhibitory constituents from Siegesbeckia pubescens.
    Article Snippet: Accepted Manuscript Nitric Oxide Inhibitory Constituents from Siegesbeckia pubescens Hari Jang, Jin Woo Lee, Jun Gu Kim, Hye Ryoung Hong, Thi Phoung Linh Le, Jin Tae Hong, Youngsoo Kim, Mi Kyeong Lee, Bang Yeon Hwang PII: S0045-2068(18)30248-7 DOI: https://doi.org/10.1016/j.bioorg.2018.05.022 Reference: YBIOO 2375 To appear in: Bioorganic Chemistry Received Date: 12 March 2018 Revised Date: 21 May 2018 Accepted Date: 22 May 2018 Please cite this article as: H. Jang, J.. Woo Lee, J. Gu Kim, H. Ryoung Hong, T. Phoung Linh Le, J. Tae Hong, Y. Kim, M. Kyeong Lee, B. Yeon Hwang, Nitric Oxide Inhibitory Constituents from Siegesbeckia pubescens, Bioorganic Chemistry (2018), doi: https://doi.org/10.1016/j.bioorg.2018.05.022 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

    Article Title: Molecular Networking-Guided Isolation of Cycloartane-type Triterpenoids from Curculigo orchioides and Their Inhibitory Effect on Nitric Oxide Production
    Article Snippet: Cell viability of the remaining cells was determined by a MTT (Sigma Chemical Co., St. Louis, MO)-based colorimetric assay.

    Colorimetric Assay:

    Article Title: Pentacyclic triterpenes with nitric oxide inhibitory activity from Potentilla chinensis.
    Article Snippet: Three new ursane-type triterpenes (1–3) and twenty-one known triterpenoids (4–24) were isolated from the methanolic extract of the whole plants of Potentilla chinensis.. Their structures were elucidated by extensive spectroscopic analysis of 1D and 2D NMR (HSQC, HMBC, COSY and ROESY) and HRESIMS data.. The bioassay screening revealed the inhibitory effects on nitric oxide production of compounds 2, 5, 7, 9, 10, and 13–24 in LPS-induced RAW264.7 macrophages.

    Article Title: Jatrophane and ingenane-type diterpenoids from Euphorbia kansui inhibit the LPS-induced NO production in RAW 264.7 cells
    Article Snippet: Accepted Manuscript Jatrophane and ingenane-type diterpenoids from Euphorbia kansui inhibit the LPS-induced NO production in RAW 264.7 cells Jin Woo Lee, Qinghao Jin, Hari Jang, Dongho Lee, Sang Bae Han, Youngsoo Kim, Jin Tae Hong, Mi Kyeong Lee, Bang Yeon Hwang PII: S0960-894X(16)30520-0 DOI: http://dx.doi.org/10.1016/j.bmcl.2016.05.035 Reference: BMCL 23894 To appear in: Bioorganic & Medicinal Chemistry Letters Received Date: 4 January 2016 Revised Date: 29 April 2016 Accepted Date: 12 May 2016 Please cite this article as: Lee, J.W., Jin, Q., Jang, H., Lee, D., Han, S.B., Kim, Y., Hong, J.T., Lee, M.K., Hwang, B.Y., Jatrophane and ingenane-type diterpenoids from Euphorbia kansui inhibit the LPS-induced NO production in RAW 264.7 cells, Bioorganic & Medicinal Chemistry Letters (2016), doi: http://dx.doi.org/10.1016/j.bmcl.. 2016.05.035 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

    Article Title: Chemical constituents from Belamcanda chinensis and their inhibitory effects on nitric oxide production in RAW 264.7 macrophage cells.
    Article Snippet: Belamcanda chinensis (L.) DC., which belongs to the family of Iridaceae, has been used as a folk medicine for the treatment of inflammation, asthma, tonsillitis, and many other throat disorders.. Bioactivity-guided purification of the methylene chloride-soluble fraction of the rhizomes of B. chinensis based on the inhibition of nitric oxide production led to the identification of seventeen known compounds.. Their structures were elucidated on the basis of extensive spectroscopic measurement such as NMR and ESI–MS.

    Article Title: Amaryllidaceae Alkaloids Screen Unveils Potent Anticoronaviral Compounds and Associated Structural Determinants.
    Article Snippet: Cell viability was monitored with the Cell-Titer GLO assay kit (Promega) or the MTT-based colorimetric assay (Cell proliferation Kit I, Roche, Millipore Sigma), where indicated.

    Article Title: Inhibitory constituents of Sophora tonkinensis on nitric oxide production in RAW 264.7 macrophages
    Article Snippet: Bioactivity-guided fractionation of the MeOH extract from the roots of Sophora tonkinensis resulted in the isolation of a new pterocarpan glycoside (1) together with nine known compounds, maackiain (2), sophoranone (3), sophoranochromene (4), pinoresinol (5), syringaresinol (6), medioresinol (7), 40 ,7-dihydroxyflavone (8), calycosin (9), and genistein (10).. The structure of the new compound was determined on the basis of spectroscopic analysis including NMR and CD data in combination with acid hydrolysis.. Compounds 1–4 exhibited the inhibitory effects on LPS-induced nitric oxide production with IC50 values ranging from 13.6 to 33.0 lM in RAW 264.7 macrophages.

    Article Title: Nitric oxide inhibitory constituents from Siegesbeckia pubescens.
    Article Snippet: Accepted Manuscript Nitric Oxide Inhibitory Constituents from Siegesbeckia pubescens Hari Jang, Jin Woo Lee, Jun Gu Kim, Hye Ryoung Hong, Thi Phoung Linh Le, Jin Tae Hong, Youngsoo Kim, Mi Kyeong Lee, Bang Yeon Hwang PII: S0045-2068(18)30248-7 DOI: https://doi.org/10.1016/j.bioorg.2018.05.022 Reference: YBIOO 2375 To appear in: Bioorganic Chemistry Received Date: 12 March 2018 Revised Date: 21 May 2018 Accepted Date: 22 May 2018 Please cite this article as: H. Jang, J.. Woo Lee, J. Gu Kim, H. Ryoung Hong, T. Phoung Linh Le, J. Tae Hong, Y. Kim, M. Kyeong Lee, B. Yeon Hwang, Nitric Oxide Inhibitory Constituents from Siegesbeckia pubescens, Bioorganic Chemistry (2018), doi: https://doi.org/10.1016/j.bioorg.2018.05.022 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

    Article Title: Molecular Networking-Guided Isolation of Cycloartane-type Triterpenoids from Curculigo orchioides and Their Inhibitory Effect on Nitric Oxide Production
    Article Snippet: Cell viability of the remaining cells was determined by a MTT (Sigma Chemical Co., St. Louis, MO)-based colorimetric assay.



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